Coding

Part:BBa_K2271145

Designed by: Jan Maika   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


Bacteriorhodopsin

This part consists of the Pex3-N-Terminal-Peroxisomal-membrane-anchor from S. cerevisiae fused to the N-terminus of the protein Bacteriorhodopsin. Bacteriorhodopsin is a proton pump from the organism Halobacterium salinarum and we used this part trying to acidify the peroxisomal lumen. The construct was designed to integrate the Bacteriorhodopsin via the Pex3-anchor into the peroxisomal membrane. The fusion protein is 768bp long, with the Pex3 anchor donating the first 48bp and Bacteriorhodopsin the later 720bp.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None